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er inhibitor ici  (Tocris)


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    Tocris er inhibitor ici
    Er Inhibitor Ici, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1070 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ers+inhibitor+ici+182+780+ici/ICI+182%2C780/pm35181438-59-1-8
    Average 96 stars, based on 1070 article reviews
    er inhibitor ici - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: Estradiol via estrogen receptor beta influences ROS levels through the transcriptional regulation of SIRT3 in human seminoma TCam-2 cells.
    Article Snippet: The specific ERs inhibitor ICI 182,780 (ICI) was purchased from Tocris Bioscience (cat. no. 1047).

    Article Title: Estrogen receptor beta (ERβ) produces autophagy and necroptosis in human seminoma cell line through the binding of the Sp1 on the phosphatase and tensin homolog deleted from chromosome 10 (PTEN) promoter gene.
    Article Snippet: The specific Estrogen Receptor (ER) β agonist Diarylpropionitrile (DPN) and the specific ERs inhibitor ICI 182,780 (ICI) were purchased from Tocris chemical (Tocris bioscience, 1494 and 1047, respectively).



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    Figure 2. Experimental protocol. Behavioral tests were performed 1 and 24 h after the last drug administration, and tissues were collected the next day. i.c.v., intracerebroventricular; ICI, ICI182780; i.g., intragastric gavage; s.c., subcutaneous.

    Journal: Molecular medicine reports

    Article Title: Saikosaponin‑D improves fear memory deficits in ovariectomized rats via the action of estrogen receptor‑α in the hippocampus.

    doi: 10.3892/mmr.2019.10232

    Figure Lengend Snippet: Figure 2. Experimental protocol. Behavioral tests were performed 1 and 24 h after the last drug administration, and tissues were collected the next day. i.c.v., intracerebroventricular; ICI, ICI182780; i.g., intragastric gavage; s.c., subcutaneous.

    Article Snippet: The non-selective estrogen receptor (er) inhibitor ici182780 (cat. no. 1047/1; Tocris Bioscience) was first dissolved in dimethyl sulfoxide (10 mM; cat. no. d103273; Shanghai Aladdin Biochemical Technology Co., Ltd.) and then diluted to 0.1% by volume in artificial cerebrospinal fluid (acSF).

    Techniques:

    Figure 4. E2 levels in the hippocampal CA1 region among the groups. E2 levels in the hippocampus were measured with an E2 ELISA kit. Data are presented as the means ± SEM. *P<0.05; **P<0.01, one‑way analysis of vari- ance and Fisher's least significant difference post hoc test. E2, estradiol; ICI, ICI182780; O, ovariectomized; SSD, saikosaponin‑D.

    Journal: Molecular medicine reports

    Article Title: Saikosaponin‑D improves fear memory deficits in ovariectomized rats via the action of estrogen receptor‑α in the hippocampus.

    doi: 10.3892/mmr.2019.10232

    Figure Lengend Snippet: Figure 4. E2 levels in the hippocampal CA1 region among the groups. E2 levels in the hippocampus were measured with an E2 ELISA kit. Data are presented as the means ± SEM. *P<0.05; **P<0.01, one‑way analysis of vari- ance and Fisher's least significant difference post hoc test. E2, estradiol; ICI, ICI182780; O, ovariectomized; SSD, saikosaponin‑D.

    Article Snippet: The non-selective estrogen receptor (er) inhibitor ici182780 (cat. no. 1047/1; Tocris Bioscience) was first dissolved in dimethyl sulfoxide (10 mM; cat. no. d103273; Shanghai Aladdin Biochemical Technology Co., Ltd.) and then diluted to 0.1% by volume in artificial cerebrospinal fluid (acSF).

    Techniques: Enzyme-linked Immunosorbent Assay

    Figure 3. Context‑dependent freezing time (%) among groups. Data are presented as the means ± SEM. *P<0.05; **P<0.01, one‑way analysis of variance and Fisher's least significant difference post hoc test. E2, estradiol; ICI, ICI182780; O, ovariectomized; SSD, saikosaponin‑D.

    Journal: Molecular medicine reports

    Article Title: Saikosaponin‑D improves fear memory deficits in ovariectomized rats via the action of estrogen receptor‑α in the hippocampus.

    doi: 10.3892/mmr.2019.10232

    Figure Lengend Snippet: Figure 3. Context‑dependent freezing time (%) among groups. Data are presented as the means ± SEM. *P<0.05; **P<0.01, one‑way analysis of variance and Fisher's least significant difference post hoc test. E2, estradiol; ICI, ICI182780; O, ovariectomized; SSD, saikosaponin‑D.

    Article Snippet: The non-selective estrogen receptor (er) inhibitor ici182780 (cat. no. 1047/1; Tocris Bioscience) was first dissolved in dimethyl sulfoxide (10 mM; cat. no. d103273; Shanghai Aladdin Biochemical Technology Co., Ltd.) and then diluted to 0.1% by volume in artificial cerebrospinal fluid (acSF).

    Techniques:

    Figure 5. ERα and ERβ expression in the hippocampal CA1 region among groups. (A and B) Immunohistochemical staining. Sections demonstrated that in the hippocampal CA1 region, ERα was present in extranuclear and nuclear sites, whereas ERβ was present in extranuclear sites. (a) The red box represents a higher magnification, and the black triangle indicates an ERα‑positive neuron; (b) the red box represents a higher magnification, and the black triangle indicates an ERβ positive neuron. (C and D) Western blot analysis for semi‑quantitative analysis of ERα and ERβ expression, whose bands were 66 and 59 kDa, respectively. The optical density of ERα or ERβ was normalized to GAPDH. Data are presented as the means ± SEM. *P<0.05; **P<0.01, one‑way analysis of variance and Fisher's least significant difference post hoc test. Scale bars: (A and B) 100 µm; (a and b) 10 µm. E2, estradiol; ER, estrogen receptor; ICI, ICI182780; O, ovariectomized; SSD, saikosaponin‑D.

    Journal: Molecular medicine reports

    Article Title: Saikosaponin‑D improves fear memory deficits in ovariectomized rats via the action of estrogen receptor‑α in the hippocampus.

    doi: 10.3892/mmr.2019.10232

    Figure Lengend Snippet: Figure 5. ERα and ERβ expression in the hippocampal CA1 region among groups. (A and B) Immunohistochemical staining. Sections demonstrated that in the hippocampal CA1 region, ERα was present in extranuclear and nuclear sites, whereas ERβ was present in extranuclear sites. (a) The red box represents a higher magnification, and the black triangle indicates an ERα‑positive neuron; (b) the red box represents a higher magnification, and the black triangle indicates an ERβ positive neuron. (C and D) Western blot analysis for semi‑quantitative analysis of ERα and ERβ expression, whose bands were 66 and 59 kDa, respectively. The optical density of ERα or ERβ was normalized to GAPDH. Data are presented as the means ± SEM. *P<0.05; **P<0.01, one‑way analysis of variance and Fisher's least significant difference post hoc test. Scale bars: (A and B) 100 µm; (a and b) 10 µm. E2, estradiol; ER, estrogen receptor; ICI, ICI182780; O, ovariectomized; SSD, saikosaponin‑D.

    Article Snippet: The non-selective estrogen receptor (er) inhibitor ici182780 (cat. no. 1047/1; Tocris Bioscience) was first dissolved in dimethyl sulfoxide (10 mM; cat. no. d103273; Shanghai Aladdin Biochemical Technology Co., Ltd.) and then diluted to 0.1% by volume in artificial cerebrospinal fluid (acSF).

    Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot

    Figure 6. Neuron numbers in the hippocampal CA1 region among the groups. (A‑G) Micrographs of Nissl‑labeled slices. (H) Histogram of the neuron number. There was no significant difference in neuron number among the groups. Scale bars: 50 µm. E2, estradiol; ER, estrogen receptor; ICI, ICI182780; O, ovariectomized; SSD, saikosaponin‑D.

    Journal: Molecular medicine reports

    Article Title: Saikosaponin‑D improves fear memory deficits in ovariectomized rats via the action of estrogen receptor‑α in the hippocampus.

    doi: 10.3892/mmr.2019.10232

    Figure Lengend Snippet: Figure 6. Neuron numbers in the hippocampal CA1 region among the groups. (A‑G) Micrographs of Nissl‑labeled slices. (H) Histogram of the neuron number. There was no significant difference in neuron number among the groups. Scale bars: 50 µm. E2, estradiol; ER, estrogen receptor; ICI, ICI182780; O, ovariectomized; SSD, saikosaponin‑D.

    Article Snippet: The non-selective estrogen receptor (er) inhibitor ici182780 (cat. no. 1047/1; Tocris Bioscience) was first dissolved in dimethyl sulfoxide (10 mM; cat. no. d103273; Shanghai Aladdin Biochemical Technology Co., Ltd.) and then diluted to 0.1% by volume in artificial cerebrospinal fluid (acSF).

    Techniques: